ABSTRACT
Objective The purpose of this study was to determine the relationship between Bcl-2 and p53 gene protein expression and radiation sensitivity according to the biologic character of laryngeal carcinoma.Methods Using monoclonal antibody for Bcl-2 and p53 gene protein,70 cases of laryngeal carcinoma were stained by immunohistochemical DACO CSA System method.Bcl-2 and p53 gene protein expression were divided into 3 levels:intense,moderate and negative expression.The relationship between expression intensity and radiation sensitivity was tested with Cramer method.Results There was relationship between each gene protein expression and radiation sensitivity.The relationship between cooperative expression and radiation sensitivity was higher than that of individual expression.Conclusion There was significant relationship between Bcl-2 and p53 gene protein coocperative expression and radiation sensitivity.The Bcl-2 and p53 gene protein cooperative expression can be consult criteria in predicting prognosis of radiation therapy of laryngeal carcinoma and instructing clinic therpy.
ABSTRACT
BACKGROUND AND OBJECTIVES: Histones, a set of highly cationic proteins essentially involved in the binding and packing of DNA in the cell nucleus chromatin, have five subclasses (H1, H2a, H2b, H3, H4) in mammalian animals. These components play the most important role in producing autoantibody in SLE and etc. Some studies proposed that there were a relationship between the activity of the disease and the titer of these autoantibody. Recently, histones were revealed to be involved in the immune deposit on GBM in MRL/lpr mice, and cause immune-mediated glomerulonephritis in normal animals. MATERIALS AND METHODS: We examined IgG deposition on the basement membrane of strial capillaries and recorded the endocochlear potential from the basal turn by an electrode inserted though the round window in guinea pigs which was injected by histone(H2a) and anti-histone antisera. RESULTS: IgG depositions were seen on the basement membrane of stria capillaries. IgG was also found to be accumulated on the extravascular side of the basement membrane. However, C3 was almost never observed in the stria vascularis of histone and the anti-histone antisera injected group. In these animals, intracellular edema was evident in the stria vascularis especially at the second and more apical turns but no inflammatory cell infiltration was present. The signifcant decrease in EP was observed just after the injection of anti-histone antisera in the animals receiving an intra-arterial injection of histones. CONCLUSION: This study indicated that cationic antigen could be trapped on the negatively charged basement membrane of strial capillaries, leading to the in situ immune complex formation, and eventually causing immune-mediated hearing loss.
Subject(s)
Animals , Mice , Antigen-Antibody Complex , Basement Membrane , Capillaries , Cell Nucleus , Chromatin , DNA , Ear, Inner , Edema , Electrodes , Glomerulonephritis , Guinea Pigs , Hearing Loss , Histones , Immune Sera , Immunoglobulin G , Injections, Intra-Arterial , Plants , Stria VascularisABSTRACT
In our daily clinical practice, we have come across several patients suffering from a fluctuating sensorineural hearing loss of unknown origin. Such patients were sometimes treated with sucess by steroid administration. Some kinds of immune injury in the inner ear have been proposed as a possible pathogenesis. However, the minute mechanisms of immune injury in the inner ear and the way in which steroids improve the hearing loss remained obscure. We have previously reported that the MRL/MP-lpr/lpr(MRL/lpr) autoimmune strain of mice showed IgG deposition in the stria vascularis with elevated thresholds of auditory brainstem response. This strain of mice though to be a good model for studying immune mediated inner ear dysfunction. The purpose of this study was to evaluated the therapeutic efffects of prednisolone on the hearing impairment in MRL/lpr mice, morphologically and electrophysiologically.